bromophenol blue การใช้

• Bromophenol blue and orange G can also be used for this purpose.
• The most common dyes are composed of bromophenol blue or xylene cyanol.
• Bromophenol blue solution can be used for the same purpose.
• A very common tracking dye is Bromophenol blue.
• Less commonly used dyes include Cresol Red and Orange G which migrate ahead of bromophenol blue.
• Commonly used color markers include Bromophenol blue, Cresol Red, Orange G and Xylene cyanol.
• Bromophenol blue and xylene cyanol may cause cancer if a person is exposed to them for too long.
• Xylene cyanol ( light blue color ) comigrates large DNA fragments, while Bromophenol blue ( dark blue ) comigrates with the smaller fragments.
• An acid-base indicator such as bromophenol blue is added to make visible the boundary between the acidic HCl solution and the near-neutral CdCl 2 solution.
• Xylene cyanol and Bromophenol blue are common dyes found in loading buffers; they run about the same speed as DNA fragments that are 5000 bp and 300 bp in length respectively, but the precise position varies with percentage of the gel.
• Generally speaking, Orange G migrates faster than bromophenol blue, which migrates faster than xylene cyanol, but the apparent " sizes " of these dyes ( compared to DNA molecules ) varies with the concentration of agarose and the buffer system used.
• For instance, in a 1 % agarose gel made in TAE buffer ( Tris-acetate-EDTA ), xylene cyanol migrates at the speed of a 3000 base pair ( bp ) molecule of DNA and bromophenol blue migrates at 400 bp.
• Even higher levels of bromine ( > 20 ppm ) can result in the secondary conversion of bromophenol red to bromophenol blue with an even lower p " K " a, erroneously giving the impression that the water has an extremely high pH despite being dangerously low.